Biacore, T200

Manager: Yinghua Chen, PhD Contact Email: yinghua.chen@case.edu Contact Phone: 216-368-8962 Availablility: By Appointment

Major Applications:

SPR device measuring binding specificity, affinity and kinetics between molecules via label-free and real-time technique;
Measure ACTIVE concentration of biomolecules;
Supports the use of 96 and 384 well microplates;

For a typical analysis of 7 minutes per cycle, up to 384 samples per run may be processed during 48 hours of unattended operation

Major specifications:

Analysis per cycle: 2-15min

Automation: 48 hours unattended operation

Sample type: low MW drug candidates to high molecular weight proteins

Flow rate range: 1-100 µL/min

Sample compartment: temperature-controlled, 4-45 °C

Flow cell volume: 0.06 µL

Flow cell height: 40 µm

Injection volume: 2-350 µL

Association rate constant (On-rates, K_a): 10³ to 10⁷ M^-1 S^-1 (and higher for macromolecular analytes)

Dissociation rate constant (Off-rates, K_d): 10^-5 to 0.5S^-1

Molecular weight detection: >100 Da

Baseline noise: < 0.1 RU

Baseline drift: < 0.3 RU/min

Sample refractive index range: 1.33-1.39

For information on theory of Biacore T100, please visit: http://www.youtube.com/watch?v=o8d46ueAwXI